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We offer a range of ultra pure, high-quality reagents for a variety of molecular biology applications such as nucleic acid electrophoresis, nucleic acid purification and sequencing, PCR and more.

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Protein Chemistry

From immunodetection reagents to protein purification, we offer a range of high-quality reagents that are pre-qualified for your protein research needs.

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Cell Biology

We offer a range of high-purity reagents that have been optimised for various cell and tissue culture applications including antibiotics, amino acids and media.

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Electrophoresis

Fisher Bioreagents offers a wide range of reagents for Electrophoresis applications. Choosing the right reagents will allow you to achieve optimal results in your experiment. Use our quick selection guides to assist you in finding the right grades of Agarose, Buffers and Ladders.

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Agarose

Compared to polyacrylamide gel, agarose has a lower resolution but wider range of separation. Lower grades of agarose can be contaminated with other polysaccharides, salts and proteins. Such impurities can alter the gelling/melting temperature of agarose solutions or affect the ability to use the recovered nucleic acid sample in a post-electrophoresis application.

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Fisher BioReagents offers three different grades of agarose that are functionally tested and pre-qualified for specific applications. Agarose grades used in electrophoresis of nucleic acids:

Genetic Analysis Grade - Agarose that yields biologically active DNA or RNA. Testing includes enzymatic performance measurements
Molecular Biology Grade - Suitable for analytical separation of DNA or RNA
PCR Grade - The original agarose for analytical separation of PCR amplicons (<1kb)

Aragose Selection Guide

Type of Agarose	Low EEO	Low Melting	Wide Separation Range	PCR Grade
Recovery of DNA and RNA	x	x	x	x
Southern and Northern Biots	x
DNA/RNA Separation 50bp to 1kb				x
DNA/RNA Separation > 1kb	x	x	x
PCR Fragment Analysis	x	x	x	x
In-gel Reactions (ligation, transformations, PCR)
Colony Lifts	x
Available Pack Sizes	100g to 500g	25g	100g to 500g	100g
Agarose Grade	Molecular Biology	Molecular Biology	Genetic Analysis	PCR

Two buffers commonly used for DNA agarose electrophoresis are Tris-acetate with EDTA (TAE; 40mM Tris-acetate, 1mM EDTA) and Tris-borate with EDTA (TBE, 89mM Tris-borate, 2mM EDTA). TAE and TBE have different properties, which makes one more suitable than the other for a specific purpose.

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Ladders

Fisher BioReagents provides a wide range of standards, including routine DNA ladders for quick size and quality assessment as well as Fisher Bioreagents™ exACTGene™ DNA ladders that allow for quantitative analysis.

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Fisher Bioreagents™ RiboLadders™ RNA Standards

These standards can be used to assess single-stranded RNA molecules on both native and denaturing agarose gels. These unique RNA standards are lyophilized to reduce thawing-related degradation, to prolong shelf life and to ensure consistent performance.

exACTGene and Routine DNA Ladders

Ready-to-use (pre-mixed with the loading dye), room temperature, stable DNA ladders are available for all common electrophoresis applications.

Application	Size Range	Numbers of Bands	Number of Loadings
exACTGene DNA ladders are ideal for qualitative analysis, quantitative estimation, and size assessment
PCR fragment analysis	25-650bp	14	100-10uL
PCR fragment analysis, small DNA digests	25-1,000bp	12	100/10uL
Quick check of PCR or enzyme digestion results	50-2,000bp	8	100/10uL
General purpose, small DNA fragments	100-1,000bp	10	100/10uL
Fast run times, small DNA fragments	100-2,000bp	8	100/10uL
Clone identification	100-2,686bp	14	100/10uL
Large size PCR or cloning	100-2,000bp	10	100/10uL
Small or large cloning application	100-5,000bp	16	100/10uL
General purpose, large digested DNA	300-10,000bp	13	100/10uL
General purpose, wide size range	100/10,000bp	19	100/10uL
General purpose, extra-large fragments	300-24,000bp	15	100/10uL
Routine DNA ladders are designed for qualitative analysis and size assessment
Small fragments, quick size assessment	50-2,000bp	11	200/5uL
Quick size assessment of broad size range	50-10,000bp	16	200/5uL

Fisher Bioreagents

High-quality, high-purity and pre-qualified reagents that meet your life science needs, all from one source.

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Aragose Selection Guide

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Ladders

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