Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Description

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

• Two applications in one kit—One buffer to process both sample types, with only one loading step
• High recovery for fragments down to 50 bp—Average recovery from 60–90%
• Minimized elution volume of 15μL—Highly concentrated DNA
• Binding buffer with pH indicator—Ensures optimal binding conditions
• Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
• Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
• Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
• Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Specifications

• Color: pH Indicator:
  Yellow = correct conditions
  Green = pH slightly high (adjustment recommended)
  Blue = pH too high (adjustment necessary)
• Format: Mini Spin Column
• Isolation Technology: Silica Membrane Technology
• Efficiency: Typical recovery: 70–95%
• Elution Volume: Elution volume: 30–100μL
• For Use With (Equipment): QIAcube™ compatible
  TAE or TBE agarose gels, PCR
• Includes: Binding Buffer NTI (2 x 75mL), Wash Buffer NT3 Concentrate (60mL), Elution Buffer NE (125mL), NucleoSpin Gel and PCR Clean-up Columns (4 x 60)
• No. of Reactions: 240 Preps
• Quantity: 1 Kit
• Purity: A₂₆₀/A₂₈₀: 1.8–1.9
• Sample Volume: <400 μL PCR reaction mixture, <400 μg agarose gel
• Size: 50 bp to ∼20 kbp
• Test Time: 10 min/6 preps
• Yield: Up to ∼15μg DNA

Safety and Handling

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H302-Harmful if swallowed.
• H412-Harmful to aquatic life with long lasting effects.

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate