PCR and qPCR

Eppendorf™ DNA LoBind™ Tubes

Eppendorf™ DNA LoBind™ Tubes

DNA LoBind™ Tubes maximize sample recovery by significantly reducing the binding of sample to the surface. Much more protein can be recovered and enzymes remain active, so they are ideal for proteomics or fields of protein research where protein concentration tends to be very small.

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1 mL

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1 mL

Reduces PCR reaction time from 2 hours to as little as 25 minutes

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL

Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL

Reduces PCR reaction time from 2 hours to as little as 25 minutes

TaqMan® TaqMan™ Gene Expression Assay (FAM)

TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.

Fisherbrand™ 0.2mL PCR Tubes

Fisherbrand™ 0.2mL PCR Tubes

With integral “snap shut” cap

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate

Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate

Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification

Beckman Coulter™ Agencourt AMPure XP

Beckman Coulter™ Agencourt AMPure XP

Trusted and recommended reagent for size selection and cleanup steps within a variety of NGS workflows. Beckman Coulter™ Agencourt AMPure XP removes unwanted contaminants from DNA in a variety of applications including PCR, NGS, cloning, and microarrays.

Fisherbrand™ 0.2mL PCR Tube Strips

Fisherbrand™ 0.2mL PCR Tube Strips

Ideal for use in 0.2mL, 96-well V-bottom thermal cyclers.

Invitrogen™ RNase-free Microfuge Tubes

Invitrogen™ RNase-free Microfuge Tubes

These microcentrifuge tubes have a non-stick, low-binding surface and are guaranteed RNase- and DNase-free.

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It provides best in class performance including the widest quantifiable range, in both single and duplex reactions, even with challenging targets.

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free

Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.

Applied Biosystems™ Random Hexamers (50 μM)

Applied Biosystems™ Random Hexamers (50 μM)

Random Hexamers are short oligodeoxyribonucleotides of random sequence [d(N)6] that anneal to random complementary sites on a target DNA or RNA to serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase.

Eppendorf™ Tubes™ 5.0 mL

Eppendorf™ Tubes™ 5.0 mL

Use these tubes for the simple and safe processing of sample volumes up to 5.0mL. Eppendorf Tubes™ represent the perfect option for working with medium–sized sample volumes.

Applied Biosystems™ MicroAmp™ Optical 8-Cap Strips

Applied Biosystems™ MicroAmp™ Optical 8-Cap Strips

Tight seal minimizes evaporation and contamination

Thermo Scientific™ Tubes, 0.2 mL, flat cap

Thermo Scientific™ Tubes, 0.2 mL, flat cap

0.2 mL individual tubes with attached flat or dome snap shut caps, available in a variety of colors

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Thermo Scientific™ PCR Plate, 96-well, low profile, skirted

Thermo Scientific™ PCR Plate, 96-well, low profile, skirted

96-well full-skirted PCR automation compatible plate for use in PCR and qPCR applications.

Axygen™ PCR Tubes with 0.5 mL Flat Cap

Axygen™ PCR Tubes with 0.5 mL Flat Cap

Thin and consistent wall thickness for precise thermal transfer

Thermo Scientific™ PCR Plate, 96-well, low profile, non-skirted

Thermo Scientific™ PCR Plate, 96-well, low profile, non-skirted

Low profile 96-well plates for use in PCR and qPCR applications.

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Get higher sensitivity, longer PCR products and higher yields in all standard PCR applications with this enhanced Taq DNA polymerase.

TaqMan™ TaqMan™ MicroRNA Reverse Transcription Kit

TaqMan™ TaqMan™ MicroRNA Reverse Transcription Kit

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

Invitrogen™ MgCl<sub>2</sub> (1 M)

Invitrogen™ MgCl2 (1 M)

Molecular biology grade, Invitrogen 1M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.

TaqMan™ TaqMan™ Gene Expression Master Mix

TaqMan™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Thermo Scientific™ Tubes, 0.5 mL, flat cap

Thermo Scientific™ Tubes, 0.5 mL, flat cap

Minimize sample loss with the “snap shut” lids on these individual 0.5mL tubes for PCR applications.

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase (2 U/μL)

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

  spinner